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Seed storage is often accompanied by a progressive loss of germination vigour and viability. In the present study, we have used Arabidopsis thaliana (L.) Heynh. seeds as a model, and carried out differential proteomics to investigate seed vigour. In our system, based on a controlled deterioration treatment (CDT), we compared seed lots treated for different time periods up to 7 days. Germination tests showed a progressive decrease of seed vigour depending on the duration of CDT. Proteomic analyses revealed that loss in seed vigour can be accounted for by protein changes in the dry seed and by an inability of the low vigour seeds to display a normal proteome during germination. Furthermore, the CDT strongly increased the extent of protein oxidation (i.e. carbonylation), which will in turn induce a loss of functional properties of proteins and enzymes and/or enhance their susceptibility towards proteolysis. These results highlighted essential mechanisms for germinative quality such as translational capacity and mobilization of seed storage reserves.
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, 570, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 630, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology/Genomics [q-bio.GN], [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, 570, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 630, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology/Genomics [q-bio.GN], [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 16 | |
popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Average | |
influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |