Establishment of a bee collection (Hymenoptera, Apoidea) as part of a biodiversity study. In the framework of the European ALARM project, we used sampling methods to assess pollinator diversity (bees and syrphid flies) and abondance, and therefore started reference collections. Identification required killing, pinning, and adequate preparation of the insects sampled. We present a set of guidelines for the preparation of bee specimens and a protocol for processing specimens caught in liquid media. We also provide recommendations to fill out labels for collection purposes. Finally, we suggest some techniques that can help keeping a well-curated bee collection.