The insectivorous genusPinguiculaoccurs along the whole Andean mountain chain from Colombia-Venezuela in the north to Tierra del Fuego in the south with a short interruption in the Peruvian-Chilean desert range. This paper describes a new and striking species ofPinguiculathat occurs in the south-eastern part of the Amotape-Huancabamba Zone in north Peru. It grows either as a lithophyte on moist rocks or as an epiphyte onPolylepis multijugaPilg. in the wet highlands of the Cordillera Central.Pinguicula rosmarieaeCasper, Bussmann & T.Henning,sp. nov.is clearly distinguished by a basal rosette of ovate-obovate leaves spread out flat on the ground and especially by a two-partite corolla with a straight uniform tube-spur complex, two features unknown from other AndeanPinguiculaspecies. The morphological similarity toP. calyptrataKunth is discussed and the habitat and distribution ofP. rosmarieaeare characterised.