The aim of the study was to evaluate the distribution of Roekoseal Automix sealer using three different obturation techniques. Forty-five single-rooted extracted human teeth were prepared in a crown-down manner using ProFile instruments. After instrumentation the specimens were filled using Roekoseal Automix with the cold lateral condensation, Thermafil and Obtura II techniques. Each root was sectioned horizontally. Color images were obtained using a reflected-light microscope. The cross-sections were assessed by microscopy for distribution of the sealer. Root canals filled with cold lateral condensation technique had statistically significant (p = 0.0001) more sealer in the apical portion in comparison to the apical portion of the specimens obturated with Thermafil and Obtura II techniques.