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Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 26 May 2015 France English Publisher:MDPI AGJournal:Biosensors, volume 5, pages 276-287 (eissn: 2079-6374, Copyright policy )Funded by:ANR | STARLET
Authors: Szunerits, Sabine; Boukherroub, Rabah; Oancea, Florin; Dima, Stefan-Ovidiu; Gouin, Sebastien G.; Alvarez-Dorta, Dimitri; Yanguas Serrano, Aritz; +5 Authors

doi: 10.3390/bios5020276

pmid: 26018780

pmc: PMC4493549

Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion

Abstract

The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min−1), and reaches a maximum at flow rates of 30 µL·min−1 (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

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Keywords

[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, <i>Escherichia coli</i> (<i>E. coli</i>), carbohydrates, Escherichia coli (E. coli), Biosensing Techniques, Article, Bacterial Adhesion, [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Escherichia coli, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Surface Plasmon Resonance, 540, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Biomechanical Phenomena, shear force enhancement, flow rate, Gold, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, surface plasmon resonance (SPR), Mannose, TP248.13-248.65, Biotechnology

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
16
Average
Average
Top 10%
Green
gold
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