AbstractField surveys conducted in December 2021 in two regions in Portugal revealed the occurrence of the invasive plant species Solanum elaeagnifolium Cav. (silverleaf nightshade), following records from the web portal BioDiversity4All. The first population was found at Costa da Caparica, municipality of Almada (Lisbon region), in an abandoned area located in an urban environment and next to the ocean, presenting a small infestation to date (ca. 200 stems). The second population was found in an abandoned field located in an agricultural area at Cano, municipality of Sousel (Northern Alentejo region). The infestation was large (>0.5 ha) and may serve as a reservoir for future invasions in nearby arable fields and olive groves. Negative effects reported in the literature for S. elaeagnifolium include competition with native vegetation, reduces crop yields and is toxic for livestock. Therefore, eradication attempts should be applied promptly in the Costa da Caparica site to eliminate the invasive alien plant before further spread. Monitoring actions and control of the population are the measures to prevent further dispersal of the plant in agricultural land in the Cano site.