Rare remains of predominantly deep-water sharks of the families Hexanchidae, Squalidae, Dalatiidae, Centrophoridae, and Squatinidae are described from the Eocene La Meseta Formation, Seymour Island, Antarctic Peninsula, which has yielded the most abundant chondrichthyan assemblage from the Southern Hemisphere to date. Previously described representatives of Hexanchus sp., Squalus weltoni, Squalus woodburnei, Centrophorus sp., and Squatina sp. are confirmed and dental variations are documented. Although the teeth of Squatina sp. differ from other Palaeogene squatinid species, we refrain from introducing a new species. A new dalatiid taxon, Eodalatias austrinalis gen. et sp. nov. is described. This new material not only increases the diversity of Eocene Antarctic elasmobranchs but also allows assuming that favourable deep-water habitats were available in the Eocene Antarctic Ocean off Antarctica in the Eocene. The occurrences of deep-water inhabitants in shallow, near-coastal waters of the Antarctic Peninsula agrees well with extant distribution patterns.