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Rose-scented geranium (Pelargonium graveolens L.) is a highly sought-after plant known for its rose-like fragrance. To meet the increasing demand for geranium oil, efficient propagation methods are essential. This study aimed to optimize in vitro techniques for the propagation of rose-scented geranium.Successful surface sterilization was achieved using a 0.1% HgCl2 treatment, resulting in a survival rate exceeding 95%. Shoot initiation was accomplished using Murashige and Skoog (MS) medium supplemented with 1.5 mg/L BAP and 0.1 mg/L IAA, resulting in a 90% shoot induction rate. Shoot multiplication was achieved on MS medium supplemented with 1.5 mg/L BAP and 0.1 mg/L NAA. The rooting of micro-shoots was successful using a rooting medium with 0.5 mg/L IBA, resulting in 5.6 cm root length per shoot. In vitro-raised plantlets were successfully acclimatized to a mixture of cocopeat, compost, and soil, with a 100% survival rate after primary acclimatization and 75% after secondary acclimatization. These optimized protocols for surface sterilization, shoot initiation, multiplication, and rooting provide a foundation for large-scale production and commercial cultivation of rose-scented geranium. Implementing these techniques can help meet the demand for geranium oil while ensuring its sustainable supply and conservation.