Mahjoub feedstock diet (MFD) is an innovation primed for livestock feed. This innovation in feed ingredients covers efficiency, profitability, environmental footprint, animal health, and welfare. This study is the first to evaluate whether manure nutrients, ammonia emissions and milk quality were affected by feeding cows with MFD compared to regular diet. We carried out a field experiment on-farm including four dairy cows to investigate the effects of the MFD feeding system on cow health, contents of dairy products, and manure composition. The results have shown excellent cow health with antibiotic-free husbandry for several years; a privileged fatty acid profile in dairy products, i.e., a major reduction in trans-fat with increased protein content and customary amino acid profile; and finally, a high C: N ratio in odorless manure. Furthermore, our methodology of approach was within the industry's conventional cost. Our cumulative results imply the preferential health and environmental performance of the feed intake diet used in the current research. Further research is planned and ongoing to deepen understanding of MFD effect on both environment and cow/human wellbeing .