The leguminous seeds of voandzou (Vigna subterranea L.) are an important source of protein and anti-nutrients. The present study analyses nutritional and antinutrients content of 3 varieties of voandzou during storage under attacked by Callosobruchus maculatus. Three varieties of voandzou seeds with distinct colors: white cream (CM/EN/DW/14), red (CM/EN/DW/07) and black (CM/EN/DW/28) were collected from local markets and stored in flasks for 3 months. Thereafter, these seeds were sieved and weighted per batch. Chemical analysis of nutritional potential according AOAC methods and Phytic acid, Tannin, Oxalate and the trypsin inhibition activity were conducted both on clean and infested voandzou seed samples. The results showed that the CM/EN/DW/14 seed variety was the most attacked (75.4%) followed by CM/EN/DW/07 (42.6%). Nutritional properties indicate that CM/EN/DW/14 had the highest nutrient contents, highest ash and moisture content, while CM/EN/DW/28 recorded the lowest nutritional content compared to the other two samples. Concentrations of studied anti-nutritional factors were significantly different according to the color. The black color has the highest concentration of anti-nutrients content compared to the red and the white cream color. Seeds with higher anti-nutrients content had less attack rates compared to the others.