The study was carried out in the experimental field of the Department of Field Crops, Faculty of Agriculture, Tekirdağ Namık Kemal University in 2020-2021. In the study, totally 25 durum wheat genotypes (6 cultivars, 12 advanced lines, and 7 landraces) were used as experimental material. The experiment was conducted in a randomized complete block design with 3 replications. In the study, the grain quality and morpho-physiological traits such as canopy temperature, chlorophyll content, leaf area index, and plant height were investigated in some durum wheat genotypes. The canopy temperature ranged from 23.33-20.43 OC, chlorophyll content 52.53-43.17 SPAD, leaf area index 3.63-1.77, thousand-grain weight 34.67-41.83 g, protein content 14.23-16.33%, test weight 78.93-87.03 kg hl-1, semolina colour 14.88-15.63 and plant height 99.00-75.33 cm in investigated durum wheat genotypes. Hacımestan and Sorgül genotypes for canopy temperature, Atkı 2, NZFM 1 and NZFM 7 genotypes for chlorophyll content, Atkı 2, NZFM 4 and NZFM 1 genotypes for leaf area index, Kıbrıs 2 and Ionia 3 genotypes for plant height, NZFM 1, NZFM 7 and Devedişi 2 genotypes for thousand-grain weight, Atkı 2 and Hacımestan 2 genotypes for protein content, Japiga and Boğacak 2 genotypes for test weight and Japiga, Boğacak, Kızıltan 91 and NZFM 7 genotypes for semolina colour were determined as promising genotypes.