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Reconstruction of prokaryotic genomes from ten termite gut metagenomes using two distinct workflows: SnakeMAGs and ATLAS.

Authors: Nachida Tadrent; Franck Dedeine; Vincent Hervé;

Reconstruction of prokaryotic genomes from ten termite gut metagenomes using two distinct workflows: SnakeMAGs and ATLAS.

Abstract

{"references": ["Kieser S, Brown J, Zdobnov EM, Trajkovski M, McCue LA. ATLAS: a Snakemake workflow for assembly, annotation, and genomic binning of metagenome sequence data. BMC Bioinformatics. 2020 Dec 22;21(1):257.", "Waidele, L., Korb, J., Voolstra, C. R., Dedeine, F., & Staubach, F. (2019). Ecological specificity of the metagenome in a set of lower termite species supports contribution of the microbiome to adaptation of the host. Animal Microbiome, 1(1), 1\u201313", "Tokuda, G., Mikaelyan, A., Fukui, C., Matsuura, Y., Watanabe, H., Fujishima, M., & Brune, A. (2018). Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites. Proceedings of the National Academy of Sciences of the United States of America, 115(51), E11996\u2013E12004", "Romero Victorica, M., Soria, M. A., Batista-Garc\u00eda, R. A., Ceja-Navarro, J. A., Vikram, S., Ortiz, M., Onta\u00f1on, O., Ghio, S., Mart\u00ednez-\u00c1vila, L., Quintero Garc\u00eda, O. J., Etcheverry, C., Campos, E., Cowan, D., Arneodo, J., & Talia, P. M. (2020). Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes. Scientific Reports, 10(1), 1\u201314", "Moreira, E. A., Persinoti, G. F., Menezes, L. R., Paix\u00e3o, D. A. A., Alvarez, T. M., Cairo, J. P. L. F., Squina, F. M., Costa-Leonardo, A. M., Rodrigues, A., Sillam-Duss\u00e8s, D., & Arab, A. (2021). Complementary Contribution of Fungi and Bacteria to Lignocellulose Digestion in the Food Stored by a Neotropical Higher Termite. Frontiers in Ecology and Evolution, 9(April), 1\u201312", "Calusinska, M., Marynowska, M., Bertucci, M., Untereiner, B., Klimek, D., Goux, X., Sillam-Duss\u00e8s, D., Gawron, P., Halder, R., Wilmes, P., Ferrer, P., Gerin, P., Roisin, Y., & Delfosse, P. (2020). Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes. Communications Biology, 3(1), 1\u201312)"]}

SnakeMAGs (Nachida Tadrent, Franck Dedeine, Vincent Hervé (Submitted). SnakeMAGs: a simple, efficient, flexible and scalable workflow to reconstruct prokaryotic genomes from metagenomes. https://doi.org/10.5281/zenodo.7303463; https://github.com/Nachida08/SnakeMAGs) is a workflow for building MAGs (Metagenome Assembled Genomes) from raw Illumina metagenomic reads. During the test phase of the development of this tool, a comparative analysis with another workflow called ATLAS v2.9.1 (Kieser et al, 2020) was performed. To compare these two workflows, we analyzed ten publicly available termite gut metagenomes (accession numbers: SRR10402454; SRR14739927; SRR8296321; SRR8296327; SRR8296329; SRR8296337; SRR8296343; DRR097505; SRR7466794; SRR7466795) from five different studies : Waidele et al, 2019; Tokuda et al, 2018; Romero Victorica et al, 2020; Moreira et al, 2021; and Calusinska et al, 2020. In this repository, we provide the configuration files that were used to launch each of the workflows (SnakeMAGs_config.yaml and ATLAS_config.yaml), as well as the obtained results, i.e. the MAGs reconstructed from each metagenome and their taxonomic classification.

Keywords

Microbiota, Termite gut, Metagenomics, MAGs, SnakeMAGs

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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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