ABSTRACT The goal of this study was to detect the most suitable treatment for multiplication of Paulownia kawakamii hybrid (101) trees to get rapid, healthy and normal plants. Shoot proliferation was induced using stem nodal explants cultured on MS, WPM or B5 medium containing different concentrations of BAP or Kin ± IBA. BAP was more suitable for inducing new shoots than Kin. The maximum numbers of the formed shoots per explant (8.3, 5.6 and 5.5) during the three successive subcultures were recorded for MS medium augmented with 1.0 mg/L BAP+0.2 mg/L IBA, 0.5 mg/L BAP+0.1 mg/L IBA and 0.5 mg/LBAP+0.1 mg/L IBA for the first, second and third subculture respectively. Key words: Paulownia, in vitro culture, plant growth regulators. Abbreviations: BAP (benzyl adenine purine), Kin (kinetin ) and IBA (indole butyric acid ). PGR (Plant growth regulator) REFERENCES Ayan, S.; Gercek, V. and Sahin, A. (2003). Performation of nursery stage of Paulownia Sieb.& Zucc. Species and Origins, Review of Faculty of Forestry University of Suleyman Demirel, Seri. A, 2: 41-56, Isparta, Turkey. Bergmann, B.A.and Moon, H.K. (1997). In vitro adventitious shoot production in Paulownia. Plant Cell Reports, 16(5): 315–319 Borchetia, S.; Das, S.C.; Handique, P.J. and Das, S. (2009). “High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.),” Scientia Horticulturae, 120 (4): 544–550. Clapa, D.; Alexanru, F.I.R.A.; Manuela, S.I.M.U. and Laura, B. (2014). Improved in vitro propagation of Paulownia elongata, P. fortunei and its interspecific hybrid P. elongata x P. fortune. Bulletin UASVM Horticulture, 7 (1): 6 – 14. Ding, J.Q.; Reardon, R.; Wu, Y.; Zheng, H. and Fu, W.D. (2006). Biological control of invasive plants through collaboration between China and the United States of America: a perspective. Biological Invasions, 8: 1439-1450. El-Showk, S., and El-Showk, N. (2003). The Paulownia tree – An alternative for sustainable forestry. The Farm. http://www.cropdevelopment.org/paulownia/Brochure. pdf. Essl, F. (2007). From ornamental to detrimental? The incipient invasion of Central Europe by Paulownia tomentosa. Preslia, 79: 377-389. Fan Guo-qiang;, Zhi Xiao-qiao, Zhai Cui-juan, BI Hui-tao, (2001). Callus induction from laves of different Paulownia species and its plantlet regeneration. Journal of forestry Research, 12 (4): 209-214. Gamborg, O.L.; Miller, R.A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 50: 151-158. Ipekçi, Z. and Gozukirmizi, N., (2003) Direct somatic embryogenesis and synthetic seed production from Paulownia elongata. Plant Cell Reports, 22(1): 16–24. Jagannathan. (1986). Phenotypic and ploidy status of Paulownia tomentosa trees regenerated from culture hypocotyls. Plant cell, Tissue and organ culture 7: 227-236. Lloyd, G. and McCown B., 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Proc. Intl. Plant Prop. Soc., 30: 421-427. Lobna S., 2008, A micropropagation protocol of Paulownia kawakamii through in vitro culture technique. Australian Journal of basic and applied sciences, 2 (3): 594-600 Murashige, T. and Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497. Nguyen M. U., Thai Xuan Du, Bui Van Le, 2005. Effect of plant growth regulators on callus induction and shoot regeneration of Paulownia fortunei. Tap chi Cong nghe Sinh hoc 3 (4): 479-485. Ozaslan M. (2005). Effect of explant source on in vitro propagation of Paulownia tomentosa Steud. Biotechnol. & Biotechnol. Eq. 19 (3): 20-26. Preston, D. (1983). Paulownia: a miracle tree or passing fancy? Am. Forests. 89(5): 15-19, 47-52. Rao, C.D.; Goh, C. J. and Kumar, P.P. (1996). High frequency adventitious shoot regeneration from excised leaves of Paulownia Spp. cultivated in vitro. Plant Cell Reports, 16: 204-209. Steel, R.G.D. and Torrie, J.H. (1980). Principle of Statistics. A Biometrical Approach 2nd, MC Graw-Hillkogakusha, Ltd. Zhu, Z.H.; Chao, C.J.; Lu, X.Y. and Xiong, Y.G., (1988). Paulownia in China: Cultivation and utilization. Singapore: Asian Network for Biological Science and International Development Research Centre of Canada.