The alpine grassland of Gran Paradiso National Park (Italy) resulted from centuries of human activities that created high biodiversity semi-natural areas below the timberline. The progressive abandonment of management practices as well as climate change lead to variations in species diversity. In order to apply active management actions for maintaining such ecosystem a long-term monitoring programme started in 2016. We will present the first results of spatial and temporal variability of CO2 fluxes from grasslands in relation to meteo-climatic conditions, together with soil properties.