Based on the principal results of the H2020 UNISECO project and roundtable debate of a panel of experts at the final project conference in March 2021, this brief formulates recommendations or future research needs and actions within a future European R&I partnership on agroecology living labs and research infrastructures. This Policy Brief is one of a set produced by the H2020 UNISECO project. Others are accessible on the Zenodo repository under the UNISECO Community. UNISECO is a European research project aiming to develop innovative approaches to enhance the understanding of socio-economic and policy drivers and barriers for further development and implementation of agro-ecological practices in EU farming systems. Learn more about the project: https://uniseco-project.eu/ This project has received funding from the European Union's H2020 research and innovation programme under grant agreement No 773901. This publication reflects only the authors' view and the European Commission is not responsible for any use that may be made of the information it contains.