There is evidence that prebiotics can impart a range of health benefits if consumed on a regular basis. With a growing market for prebiotic-containing foods there is increasing interest in understanding how prebiotics function at the molecular level. Advances in the genomics of lactobacilli and bifidobacteria have enabled modeling of transport and catabolic pathways for prebiotic utilization. The goal of this study was to develop a physiological model of the upper gastrointestinal tract to investigate the metabolic profile of probiotic strain Lactobacillus bulgaricus L14 in present of 0.75% polysaccharides obtained from Plantago major L. leaves. The polysaccharide degradation and utilization from probiotic strain L14 using in vitro model including gastric phase and intestinal phase simulated digestion in humans was developed.

Acknowledgments: We would like to express our gratitude for the support of this work by the project BG05M20P001-1.002-0005, Personalized Innovative Medicine Competence Center (PERIMED), operational program "Science and education for smart growth" 2014-2020.