In addition to alfalfa, red clover (Trifolium pratense L.) is the most important perennial forage legume grown in Serbia. It is rich in proteins that are important for the nutrition of livestock. Red clover, like many legumes, is parasitized by fungi of the genus Colletotrichum, and the consequence is significant economic damage in the form of reduced yields and reduced hay quality. In this study, sixteen cultivars of red clover of different geographical origin were tested for the resistance to the isolate Coll-44 (C. linicola). The susceptibility of different cultivars of red clover to the tested C. linicola isolate was determined using the scale of 1-5. Six cultivars (Wilo, Repio, Viglana, Kolubara, K-39 and K-17) showed high levels of resistance to the tested isolate of C. linicola. Based on the severity index, the most resistant to the isolate of C. linicola was the American variety Wilo with a severity index of 1.6 (50%), while the lowest severity index of the Serbian variety K-39 was 2.6 (20%). The purpose of this research was to evaluate the susceptibility and tolerance level of 16 red clover cultivars to anthracnose causative agents, in this case, C. linicola.