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Article . 1999
License: CC BY NC ND
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Mesure Du Nadh Par Microchip Yag Triplé

Authors: Sirgant, Aline; Mottin, Stéphane; Cespuglio, Raymond; Pedri, Claude; Laporte, Pierre;

Mesure Du Nadh Par Microchip Yag Triplé

Abstract

Nous présentons une instrumentation compacte, transportable, développé à l origine pour mesurer la fluorescence induite par laser du NADH cérébral chez l animal libre de ces mouvements. La source laser microchip Nd3+ : YAG est triplée en fréquence. Une fibre optique amène la lumière laser jusqu à la zone cible du cerveau et cette même fibre récupère la fluorescence de cette microzone (noyau du raphé dorsal). L acquisition de données se fait via un monochromateur suivi soit d un photomultiplicateur et d un Box-car soit d une caméra à balayage de fente. Les premières expériences réalisées sur un rat non anesthésié ou in vitro avec les deux systèmes d acquisition nous ont permis de vérifier que le niveau de fluorescence était suffisant pour être détecté. Les applications de ce capteur, développées actuellement, sont la détection des effets de certains médicaments au niveau du système nerveux central, l analyse des variations du NADH lors de l alternance des états de vigilance, ainsi qu une étude de l épilepsie.

License CC-BY-NC-ND. --------- French law about open access and open science: https://www.legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000033202746&categorieLien=id ----------------------- LOI n° 2016-1321 du 7 octobre 2016 pour une République numérique - Article 30.

{"references": ["D. Guillot, EuroPhotonics, Ao\u00fbt/Septembre 1997.", "St\u00e9phane Mottin, Hamamatsu Photonique Informations,14."]}

Country
France
Keywords

[PHYS]Physics [physics], Time-resolved spectroscopy, Ultrafast technology, http://id.loc.gov/authorities/subjects/sh89005705.html, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, brain, http://id.loc.gov/authorities/subjects/sh91006099.html, Optical design, Picosecond laser, laser, in vivo, http://id.loc.gov/authorities/subjects/sh85095181.html, http://id.loc.gov/authorities/subjects/sh85091159.html, [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], rat, Spectroscopy, Sensor

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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