Aloe vera Linn., plant belongs to Liliaceae family, is a plant originated in the warm, dry climates of Africa readily adaptable and grows worldwide. Slow propagation rate of axillary shoots and male sterility to use its seeds hinders its efficient propagation. Hence, the present study aimed to standardize optimum in vitro propagation conditions for it. Based on this purpose MS media supplemented with 0.6 mg/L BAP and 0.2 mg/L of IBA; 1.0 mg/L BAP; and devoid of hormone were selected as the optimum medium for Aloe vera establishment, multiplication and rooting respectively. The higher mean number of shoots, 2.90 and multiplication factor, 2.87 were registered on MS media with 0.2 mg/L IBA & 1.0 mg/L BAP respectively. During their rooting and acclimatization, the selected treatment yields 100% rooted plant and 92.59 survival percentage during acclimatization on 3:1 mixture of filter cake and sand after their fifth week. Application of this protocol helps to propagate thousands of Aloe vera plants from few initial mother plants in year period.