The authors isolated yeast strains from the surface of grape cultivars Burgund (strain I7), hops cones grown in the botanical garden of Gorsky state agrarian university (Vladikavkaz, Russia) (strain DEN4), as well as from soil samples of the molasses distillery stillage drainfields (strain B1). The primary identification of the selected strains was performed by studying cultural, tinctorial, morphological, physiological-biochemical, and technological properties in the Research Institute of Biotechnology of Gorsky SAU, using traditional research methods. For the final identification based on the analysis of ribosomal genes sequence of the studied strains, colonies on the slant wort agar were sent to the Bioresource Centre – All-Russian Collection of Industrial Microorganisms (BRC VKPM) National Research Centre “Kurchatov Institute” – State Research Institute of Genetics. According to the results of the performed in BRC VKPM NRC “Kurchatov Institute” – State Research Institute of Genetics analysis of the nucleotide sequence, coding part of rRNA genes it was found that I7 strain is the closest to the species Hanseniaspora uvarum; DEN4 strain is closest to the species Saccharomyces cerevisiae; B1 strain is closest to the species Torulaspora delbrueckii. Key Words: yeasts, strain, gene encoding 18S rRNA, DNA, PCR mode.