Perennial, no-food grasses have been proposed as the most efficient species for biomass production due to their natural resource use efficiency, agronomic, environmental and social benefits. Nitrogen requirement is a significant issue in intensive agriculture and greatly affects the energetic balance of crops. Hence, reduced nitrogen fertilizer by means of low input cropping systems could directly mitigate greenhouse gas emissions. In the present study long-term plantations of two perennial grasses (Arundo donax and Miscanthus x giganteus) grown in rainfed conditions under two nitrogen regimes were compared in terms of biomass yield and nitrogen use efficiency.