The Human intestinal Bacteria Collection (HiBC) is a collection of bacterial strains, isolated from the human gut for which 16S rRNA gene sequences, genome sequences and culture conditions are made available to the research community. In addition to previously described bacteria, we include strains that represent novel species which have been taxonomically described and validly named, or will be in the future. This collection will be updated regularly. This dataset includes the taxonomy of the isolates, as well as metadata regarding their cultivation and isolation. We also provide metadata regarding the sequencing, genome assembly process and the biological sequences. UPDATE v6: The growth atmosphere is now indicated by anaerobic or aerobic instead of "Anaerobe/Aerobe" that was a misleading term. The risk group of these two isolates went from 1 to 2: CLA-AA-H205: Anaerostipes caccae CLA-AA-H83: Bacteroides fragilis The risk group of the following isolates has been updated (usually from unknown to 1, or from 2 to 1): CLA-SR-H026: Aedoeadaptatus acetigenes CLA-KB-H139: Bacteroides xylanisolvens CLA-SR-H015: Bacteroides xylanisolvens CLA-AA-H187: Blautia fusiformis CLA-AA-H274: Brotaphodocola catenula CLA-AA-H286: Butyricimonas faecihominis CLA-AA-H278: Clostridium fessum CLA-AA-H147: Dorea ammoniilytica CLA-SR-H027: Dorea formicigenerans CLA-KB-H89: Dorea longicatena CLA-KB-H94: Dorea longicatena CLA-SR-H022: Enterococcus lactis CLA-AA-H250: Hominenteromicrobium mulieris CLA-AA-H232: Hominilimicola fabiformis CLA-AA-H246: Hominisplanchenecus faecis CLA-AA-H276: Hominiventricola filiformis CLA-AA-H213: Oliverpabstia intestinalis CLA-AA-H241: Oliverpabstia intestinalis CLA-AA-H58: Pilosibacter fragilis CLA-KB-H110: Ruthenibacterium lactatiformans CLA-AA-H174: Segatella sinensis CLA-AA-H2: Veillonella parvula CLA-AA-H273: Waltera acetigignens Typos in media list have been fixed. UPDATE v5: The accessions number for the genomes on INSDC databases are added under the column Accession. Plus two typos in the risk group column have been corrected as follow: CLA-AA-H173: from Risk Group 4 (!) to 2 like the other strain of Sutterella wadsworthensis CLA-AA-H198: from Risk Group 4 (!) to 1 like the other Bifidobacterium species. UPDATE v4: Only the taxonomy of a couple of isolates has been changed, as follow: CLA-ER-H4: Collinsella sp900547855 instead of Collinsella sp900544645 CLA-AA-H142: Pilosibacter fragilis (f__Clostridiaceae) instead of Sakamotonia hominis gen. nov. (f__Lachnospiraceae) CLA-AA-H58: Pilosibacter fragilis (f__Clostridiaceae) instead of Sakamotonia hominis gen. nov. (f__Lachnospiraceae) CLA-AA-H89B: Lachnospira intestinalis sp. nov. instead of Lachnospira hominis sp. nov. CLA-JM-H10: Lachnospira hominis sp. nov. instead of Lachnospira intestinalis sp. nov. CLA-JM-H7B: Faecalibacterium taiwanense instead of Faecalibacterium faecis sp. nov. CLA-JM-H45: Merdimmobilis hominis instead of Hominicola intestinalis gen. nov. UPDATE v3: The genome of one of our isolate had been unfortunately swapped. This mistake has been now corrected on Zenodo and Coscine. The genome of Segatella sinensis CLA-AA-H117 should be considered correct with 103 contigs and 3 671 232 nt. Please note that the genome available at the NCBI is the correct one (GCA_040324585.2). Two typos regarding taxonomy have been corrected as well: Maccoya intestinihominis has been corrected to Maccoyia intestinihominis and Faecousia faecis to Faecousia intestinalis.