AbstractWe investigated a large colony of European free-tailed bats (Tadarida teniotis) in Spain, using a combination of capture-mark-recapture data and direct observations. Its social and reproductive organisation is complex and the mating system fits a “resource defence polygyny” model. In spring and autumn, aggressive interactions in flight, defence of roosts and mating songs of males to attract females occurred. According to our results,T. teniotisis organised in “harems” consisting of a dominant male and a variable number of females. In addition the sexual cycle displayed a bimodal reproductive pattern (this is unique and remarkable for European bats). The bimodal pattern coincided with peaks in food availability (moths) at high altitudes. Presumably, roost-guarding activities (patrolling, advertising…) make males less prone to move away (e.g. at higher altitudes and longer distances) from roosts, resulting in differences in prey selection and in altitudinal segregation between sexes. This provides a plausible explanation for the differences in diet (predation of more sedentary vs. high-flying migratory moths) between males and females that has been found in published studies.