This data set contains surface reconstructions (in ply-format) of - ZO-1 (ventricular labeling) is shown in green, - mCherry positive cells (her5, CRE-induced at 24hpf) are shown in magenta, and - DiD membrane labeling is shown in transparent grey in support of figure 6. For visualization of these data, the application BrainVispy (Windows only) is co-packaged. This application is an open-source development of the Zuse-zentrum, Berlin. Details on this software can be found at https://github.com/zibneuro/brainvispy. Usage: - Download and unpack FigureS2.zip . - From the resulting folder FigureS2 start the application BrainVisPy_v5.exe by double-click (runs without installation). - In BrainVisPy open the project 14dpf-ventricles, which is located in the same folder. -- From the menu select FILE>Open project (or CTRL+O) and select 14dpfventricles.xml from the folder FigureS2 . Depending on the settings of your computer, the extensions (.exe, .xml) may not be displayed.

Since the individual surfaces are stored in ply-format, they also can be visualized in any other software capable of opening ply-files.