Teaching materials are auxiliary elements employed to accomplish the goals of the educational process. The observed fact reveals a deficiency in teaching materials that incorporate Technological Pedagogical Content Knowledge (TPACK) for the purpose of developing students’ critical thinking abilities. Thus, there is a want for an instructional resource that may assist pupils in enhancing their critical thinking abilities. The objective of this project is to provide TPACK-based teaching materials on biodiversity that are credible, effective, and practical. In addition to elucidating students’ critical thinking abilities through the utilization of TPACK-based instructional resources. The methodology employed in this study is Research and Development (R&D) utilizing the ADDIE paradigm. The tool employed is a questionnaire sheet for the purpose of validating materials and media. The study yielded data indicating that teaching materials centered around Technological Pedagogical Content Knowledge (TPACK) were valid and appropriate for learning purposes in the context of Biodiversity. The validity includes a material validation score of 91.25% and a media score of 96.42%, with an N-Gain of 0.75, indicating strong testing performance. Therefore, it can be inferred that educational resources rooted in Technological Pedagogical Content Knowledge (TPACK) and focused on the topic of Biodiversity have a positive impact on students’ ability to think critically.