This data set contains coordinate records of alien (i.e., non-native) species populations worldwide and aggregated checklists of alien species for individual regions. The regions consists of non-overlapping polygons representing countries, sub-national or coastal marine ecoregions. The data set was produced by applying the DASCO workflow (https://doi.org/10.5281/zenodo.5841930) using the SInAS database (version 2.5; https://doi.org/10.5281/zenodo.10038256). The workflow imports checklists of alien species such as those stored in SInAS, and extracts coordinates for the alien regions (according to SInAS) from GBIF and OBIS. After cleaning and thinning the coordinates, the workflow exports a list of coordinates of alien populations for all species included in SInAS and with records on GBIF or OBIS. These files are part of a manuscript published in the journal Neobiota, where the workflow is described in detail (Seebens & Kaplan 2022, https://doi.org/10.3897/neobiota.74.81082). DASCO_AlienCoordinates_SInAS_2.5.gz contains the coordinates of alien populations. DASCO_AlienRegions_SInAS_2.5.csv contains the checklists of alien species per region. Note that this only includes species with GBIF and OBIS records. For more comprehensive checklists, other databases such as those listed here (https://doi.org/10.5281/zenodo.10038256) should be consulted. OBIS_SpeciesKeys_SInAS_2.5.csv contains the species keys from OBIS. GBIF_SpeciesKeys_SInAS_2.5.csv contains the species keys from GBIF. DASCO_TaxonHabitats_SInAS_2.5.csv contains habitat information for individual species if available from WoRMS, Fishbase or Sealifebase (used to identify marine species). The file DASCO_ListOriginalGBIFData_keys_SInAS_2.5.csv contains the DOIs of the originally downloaded files from GBIF, which provides the basis for the generation of the GBIF part (ie. the DASCO workflow was applied to these data sets from GBIF). Note that OBIS does not provide a DOI for downloads, and thus we cannot provide this.