Biofuels are considered to be the most promising substitutes for the decarbonisation of the transport sector, especially heavy road and air transport. For this reason new and more sustainable drop-in biofuel alternatives are being pursued with the aim of overcoming the current challenges and limitations exhibited by 1st generation biofuels through R&D projects such as Next Generation Butanol (ButaNexT). The ButaNexT project aims to develop a highly efficient production process for the conversion of sustainable lignocellulosic feedstocks into bio-butanol through the integration of different technology developments. Begun in 2015, the project aims to develop, optimise and integrate several novel and advanced technologies for the production of sustainable, more efficient and versatile biobutanol from lignocellulosic feedstocks and wastes. The following article shows the main achievements to date of the ButaNexT project towards developing next generation biobutanol.