In this study we improved the knowledge about terrestrial mammals (excluding Chiroptera) of the 'Lago di Tarsia' Regional Nature Reserve (Special Conservation Area; Cosenza; Southern Italy), collecting a large amount both of direct and indirect original data (including road-killing remains and items obtained by small mammal traps, owl pellets, and phototraps), and bibliographic ones. We obtained 424 records belonging to 22 taxa classified at species level and one requiring a further taxonomic diagnosis (Microtus savii vel brachycerchus). Hystrix cristata Linnaeus, 1758, Vulpes vulpes (Linnaeus, 1758) and Martes sp. were the taxa with the highest number of records (> 10%). Sciurus meridionalis (Lucifero, 1907), Martes foina (Erxleben, 1777), Glis glis (Linnaeus, 1758), Apodemus cfr. flavicollis (Melchior, 1834), Lepus europaeus Pallas, 1778, Talpa romana (Thomas, 1902), Rattus cfr. rattus (Linnaeus, 1758), Arvicola amphibius (Linnaeus, 1758) were the taxa with the lowest number (<1%). Almost half of the records have been obtained by indirect traces (hairs, excrements, fingerprints, burrows, etc.). Records from owl pellets represented an important percentage. Although the study area is restricted in size, the high environmental heterogeneity make possible the co-occurrence of a high number of species, including specialized taxa (e.g., forest mammals, mustelids and Soricomorpha). Some species, occurring in the neighbouring, although not recorded in our study (e.g. Sorex spp., Neomys sp., Elyomis quercinus Linnaeus, 1766), could be confirmed improving the research effort. Further studies are necessary in this regard, also focusing on the role of this reserve as dispersal corridor for rare carnivores, occurring in the massifs and rivers neighbouring to the Reserve (e.g. Lutra lutra Linnaeus, 1758 and Canis lupus Linnaeus, 1758).