‘Blanc Du Bois’ (Vitisspp.) is the most widely grown Pierce’s disease tolerant white grapevine cultivar in Texas. As an interspecific hybrid, its growth habit is seimi-drooping, and 'Blanc Du Bois' is characterized as vigorous. This study evaluated the impact of training system (Mid-Wire Cordon with VSP, Mid-Wire Cane with VSP and Smart-Dyson, High-Wire Quadralateral, and Watson) on 'Blanc Du Bois' growth, yield components, and fruit composition at two locations in Texas. The first site was located in the Rio Grande Valley where the mean extreme minimum winter temperature is -1.1 to 1.7° C (USDA Cold Hardiness Zone 10a). As a result of climate and site conditions, vine size, determined by dormant pruning weight, was very large averaging from 3.71 to 5.56 kg per vine across training systems over a three-year period. At this site, the horizontally divided systems, High-Wire Quadrilateral and Watson were the highest yielding averaging 10.66 and 7.49 kg per vine, respectively, as a result of more shoots per vine, and higher fruitfulness. The Mid-Wire Cordon and Mid-Wire Cane Pruned Training Systems had lower yields in two out of three years, but fruit maturity indices soluble solids and pH reflected more advanced maturity at harvest. At the second site, located in the Central Gulf Coast of Texas (USDA Cold Hardiness Zone 8b), vines were less vigorous with pruning weights averaging 1.66 to 1.83 kg per vine across training systems over three years. Consistent differences in yield components, vine size, and fruit composition were not observed, and all the three training systems under study had acceptable growth and fruiting characteristics. The results of this research suggest that 'Blanc Du Bois’ vigor potential and growth habit makes it well-suited for horizontally divided canopy training systems, particularly on vigorous sites.