Amphibian and reptile diversity from Isla Santay (Durán, Ecuador) has been underestimated due to the lack of monitoring throughout the island. Historically, this island has also been affected by anthropic landscape changes which limit the occurrence of certain species and favors the dispersion of opportunistic or more tolerant species. Such species dynamics tend to be more evident on islands, since their isolation limits species’ mobility. We present an updated checklist of the herpetofauna of Isla Santay Durán, Ecuador, and report for the first time the occurrence of two introduced species, Anolis sagrei Duméril & Bibron, 1837, Brown Anole, and Hemydactylus frenatus Duméril & Bibron, 1836.