
Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant, respectively. The results showed high contamination of the bulbs. There were no significant differences between the treatments with plant growth regulators. A multiplication rate of 1.3 – 2.2 for bulbils was achieved, with an average of 28 bulbils per mother bulb. The natural regeneration rate is approximately 1 - 4 bulbils per mother bulb once a year. Traubia modesta pertenece a la familia Amaryllidaceae y es endemica de Chile, que se encuentra en la categoria de “en peligro” de extincion. En esta investigacion se desarrollo un protocolo de propagacion de la especie a traves del cultivo in vitro de organos vegetativos. Se utilizo la tecnica de escamas gemelas cultivadas en medio MS suplementado con ANA y BAP en distintas concentraciones, se adiciono PPM como biocida y PVP como compuesto antioxidante. Los resultados obtenidos muestran una alta contaminacion en los bulbos. No hubo diferencias significativas entre los tratamientos con reguladores del crecimiento vegetal. Se logro una tasa de multiplicacion de microbulbillos de 1,3 a 2,2, donde el promedio de bulbos obtenidos en todos los tratamientos por bulbo madre fue de 28. La regeneracion natural es alrededor de 1 a 4 unidades por bulbo madre una vez al ano.
Bulbing, Bulbificación, twin scales, especie en peligro de extinción, In vitro propagation, escamas gemelas, propagación in vitro, Traubia modesta, critically endangered species
Bulbing, Bulbificación, twin scales, especie en peligro de extinción, In vitro propagation, escamas gemelas, propagación in vitro, Traubia modesta, critically endangered species
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