From bud formation to flowering: transcriptomic state defines the cherry developmental phases of sweet cherry bud dormancy
Copyright policy )From bud formation to flowering: transcriptomic state defines the cherry developmental phases of sweet cherry bud dormancy
Abstract Background Bud dormancy is a crucial stage in perennial trees and allows survival over winter to ensure optimal flowering and fruit production. Recent work highlighted physiological and molecular events occurring during bud dormancy in trees. However, they usually examined bud development or bud dormancy in isolation. In this work, we aimed to further explore the global transcriptional changes happening throughout bud development and dormancy onset, progression and release. Results Using next-generation sequencing and modelling, we conducted an in-depth transcriptomic analysis for all stages of flower buds in several sweet cherry ( Prunus avium L.) cultivars that are characterized for their contrasted dates of dormancy release. We find that buds in organogenesis, paradormancy, endodormancy and ecodormancy stages are defined by the expression of genes involved in specific pathways, and these are conserved between different sweet cherry cultivars. In particular, we found that DORMANCY ASSOCIATED MADS-box ( DAM ), floral identity and organogenesis genes are up-regulated during the pre-dormancy stages while endodormancy is characterized by a complex array of signalling pathways, including cold response genes, ABA and oxidation-reduction processes. After dormancy release, genes associated with global cell activity, division and differentiation are activated during ecodormancy and growth resumption. We then went a step beyond the global transcriptomic analysis and we developed a model based on the transcriptional profiles of just seven genes to accurately predict the main bud dormancy stages. Conclusions Overall, this study has allowed us to better understand the transcriptional changes occurring throughout the different phases of flower bud development, from bud formation in the summer to flowering in the following spring. Our work sets the stage for the development of fast and cost effective diagnostic tools to molecularly define the dormancy stages. Such integrative approaches will therefore be extremely useful for a better comprehension of complex phenological processes in many species.
- University of Bordeaux France
- Max Planck Institute for Plant Breeding Research Germany
- University of Cambridge United Kingdom
- National Research Institute for Agriculture, Food and Environment France
- University of Murcia Spain
[SDV]Life Sciences [q-bio], Flowers, QH426-470, Prunus avium, 630, Gene Expression Regulation, Plant, Genetics, Prunus avium L., Prediction;Prunus avium L.;RNA sequencing;Seasonal timing;Time course;Transcriptomic, time course, Plant Proteins, Prunus avium L, Time course, Vegetal Biology, Models, Genetic, Gene Expression Profiling, Botánica, Gene Expression Regulation, Developmental, High-Throughput Nucleotide Sequencing, RNA sequencing, prediction, seasonal, Time Course, Plant Dormancy, dormance, [SDV] Life Sciences [q-bio], Rna Sequencing, arbre fruitier à noyau, Seasonal timing, Transcriptomic, Seasonal Timing, fruit à noyau, Prediction, Biologie végétale, TP248.13-248.65, amélioration des plantes, Biotechnology, Research Article
[SDV]Life Sciences [q-bio], Flowers, QH426-470, Prunus avium, 630, Gene Expression Regulation, Plant, Genetics, Prunus avium L., Prediction;Prunus avium L.;RNA sequencing;Seasonal timing;Time course;Transcriptomic, time course, Plant Proteins, Prunus avium L, Time course, Vegetal Biology, Models, Genetic, Gene Expression Profiling, Botánica, Gene Expression Regulation, Developmental, High-Throughput Nucleotide Sequencing, RNA sequencing, prediction, seasonal, Time Course, Plant Dormancy, dormance, [SDV] Life Sciences [q-bio], Rna Sequencing, arbre fruitier à noyau, Seasonal timing, Transcriptomic, Seasonal Timing, fruit à noyau, Prediction, Biologie végétale, TP248.13-248.65, amélioration des plantes, Biotechnology, Research Article
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).67 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!