In acoustic communication timing seems to be an exceedingly important aspect. The just noticeable difference (jnd) for small perturbations of an isochronous sequence of sounds is particularly important in music, in which such sequences frequently occur. This article reviews the literature in the area and presents an experiment designed to resolve some conflicting results in the literature regarding the tempo dependence for quick tempi and relevance of music experience. The jnd for a perturbation of the timing of a tone appearing in an isochronous sequence was examined by the method of adjustment. Thirty listeners of varied musical background were asked to adjust the position of the fourth tone in a sequence of six, such that they heard the sequence as perfectly isochronous. The tones were presented at a constant interonset time that was varied between 100 and 1000 ms. The absolute jnd was found to be approximately constant at 6 ms for tone interonset intervals shorter than about 240 ms and the relative jnd constant at 2.5% of the tone interonsets above 240 ms. Subjects’ musical training did not affect these values. Comparison with previous work showed that a constant absolute jnd below 250 ms and constant relative jnd above 250 ms tend to appear regardless of the perturbation type, at least if the sequence is relatively short.