The Drosophila hnRNPA/B Homolog, Hrp48, Is Specifically Required for a Distinct Step in osk mRNA Localization
Copyright policy )The Drosophila hnRNPA/B Homolog, Hrp48, Is Specifically Required for a Distinct Step in osk mRNA Localization
The Staufen-dependent localization of oskar mRNA to the posterior of the Drosophila oocyte induces the formation of the pole plasm, which contains the abdominal and germline determinants. In a germline clone screen for mutations that disrupt the posterior localization of GFP-Staufen, we isolated three missense alleles in the hnRNPA/B homolog, Hrp48. These mutants specifically abolish osk mRNA localization, without affecting its translational control or splicing, or the localization of bicoid and gurken mRNAs and the organization of the microtubule cytoskeleton. Hrp48 colocalizes with osk mRNA throughout oogenesis, and interacts with its 5' and 3' regulatory regions, suggesting that it binds directly to oskar mRNA to mediate its posterior transport. The hrp48 alleles cause a different oskar mRNA localization defect from other mutants, and disrupt the formation of GFP-Staufen particles. This suggests a new step in the localization pathway, which may correspond to the assembly of Staufen/oskar mRNA transport particles.
- UNIVERSITE PARIS DESCARTES France
- University of Cambridge United Kingdom
- French National Centre for Scientific Research France
- Wellcome Trust United Kingdom
- Institut des Sciences Biologiques France
571, Carrier proteins, Mago Nashi, Mutation, Missense, Sequence Homology, Alpha-like Protein, 0601 Biochemistry and Cell Biology, Posterior Localization, Heterogeneous-Nuclear Ribonucleoproteins, Oskar, Oogenesis, Cytoplasmic Transport, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Translational Repressor Bruno, Anterior-posterior, Animals, Drosophila Proteins, RNA, Messenger, 3' Untranslated Regions, Homeodomain Proteins, Binding Sites, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], Messenger RNA, Cell Polarity, RNA-Binding Proteins, Cell Biology, Transforming Growth Factor alpha, Pair-rule Transcripts, Cell Compartmentation, Drosophila melanogaster, Hippocampal-neurons, Localization, Cell polarity, Mutation, Oocytes, Trans-Activators, Drosophila, Axis Formation, Hrp48, Carrier Proteins, Binding Protein, Developmental Biology, Transcription Factors
571, Carrier proteins, Mago Nashi, Mutation, Missense, Sequence Homology, Alpha-like Protein, 0601 Biochemistry and Cell Biology, Posterior Localization, Heterogeneous-Nuclear Ribonucleoproteins, Oskar, Oogenesis, Cytoplasmic Transport, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Translational Repressor Bruno, Anterior-posterior, Animals, Drosophila Proteins, RNA, Messenger, 3' Untranslated Regions, Homeodomain Proteins, Binding Sites, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], Messenger RNA, Cell Polarity, RNA-Binding Proteins, Cell Biology, Transforming Growth Factor alpha, Pair-rule Transcripts, Cell Compartmentation, Drosophila melanogaster, Hippocampal-neurons, Localization, Cell polarity, Mutation, Oocytes, Trans-Activators, Drosophila, Axis Formation, Hrp48, Carrier Proteins, Binding Protein, Developmental Biology, Transcription Factors
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