In order to assess the safety of genetically modified crops, the anti-nutrients were determined in these novel crops and compared with their parental lines.Five kinds of crops (rice, maize, soybean, cottonseed and rapeseed) from domestic and foreign companies were collected and the contents of anti-nutrients for each kind were analyzed. These anti-nutrients include phytate and protease-inhibitors (in rice, maize and soybean), gossypol (in cottonseed), glucosinolates and erucic acids (in rapeseed).The contents of anti-nutrients in one type of maize, three types of rapeseeds and two types of cottonseeds didn't meet the requirement of "equivalence" in this study, but the finally conclusion should be made after many experimental data in the future.