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Article . 2017
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Aislamiento e identificación de antagonistas asociados al parasitismo de esclerocios de Sclerotinia sclerotiorum en la provincia de Tucumán, Argentina

Authors: Allori Stazzonelli, Enzo; Yasem, Marta Graciela; Ploper, Leonardo Daniel;

Aislamiento e identificación de antagonistas asociados al parasitismo de esclerocios de Sclerotinia sclerotiorum en la provincia de Tucumán, Argentina

Abstract

En el Noroeste Argentino, la producción de poroto representa una importante actividad agrícola. Uno de los problemas sanitarios más complejos en el cultivo es provocado por Sclerotinia sclerotiorum, un hongo capaz de producir esclerocios que juegan un importante rol en el ciclo de la enfermedad. El objetivo de este trabajo fue el aislamiento e identificación de antagonistas nativos asociados al parasitismo de esclerocios de S. sclerotiorum en la provincia de Tucumán, Argentina. Para el aislamiento se implementó la técnica del esclerocio trampa en 3 regiones agrológicas de la provincia y se colectaron esclerocios parasitados naturalmente. Se obtuvieron 59 aislamientos de los esclerocios trampa y 3 de esclerocios colectados en centros de comercialización de frutas y verduras. En la región del Pedemonte se aislaron la mayor cantidad de antagonistas, específicamente el 76 % del total. De acuerdo al análisis de las secuencias de la región ITS1-ITS2 y de la región que codifica para el gen del factor de elongación -1α (tef1α), se observó que la distribución de las especies de Trichoderma no es homogénea entre los suelos de las regiones evaluadas. Las especies de Trichoderma que predominaron fueron T. atroviride y T. koningiopsis, ambas pertenecientes a la sección Trichoderma, clado viride mientras que solo se identificó un aislamiento de la especie T. longibrachiatum y uno de la especie T. afroharzianum. Palabras clave: Aislamiento; Identificación; Esclerocio; Trichoderma.

In Northwestern Argentina, bean production constitutes a major agricultural activity. One of the most complex phytosanitary problems affecting this crop is caused by Sclerotinia sclerotiorum, a fungus capable of producing sclerotia that play an important part in the disease cycle. The aim of this work was to isolate and identify native antagonists associated with parasitism on Sclerotinia sclerotiorum sclerotia in Tucumán province, Argentina. The antagonists were isolated using the sclerotia bait technique, and by retrieving them from naturally parasitized sclerotia, in three agrologic regions in the province. Fifty nine isolates were obtained using sclerotia as baits and 3 were recovered from collected sclerotia at fruit and vegetable commercialization centers. Most of the antagonists, specifically 76% of them, were isolated in the foothills region (called “Pedemonte”). The analysis of sequences of the ITS1-ITS2 region and that of the region coding for thetranslationelongation factor -1α gen (tef1α) revealed that Trichoderma species do not have a homogeneous distribution in the soil types of the evaluation sites. The prevalent Trichoderma species were T. atroviride and T. koningiopsis, both of which belong to the Trichoderma sect., viride clade. Only one isolate of the T. longibrachiatum species was identified, as well as one of T. afroharzianum.

Fil: Ploper, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina

Fil: Allori Stazzonelli, Enzo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia. Sanidad Vegetal. Cátedra Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina

Fil: Yasem, Marta Graciela. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina

Country
Argentina
Keywords

ESCLEROCIO, IDENTIFICACION, AISLAMIENTO, https://purl.org/becyt/ford/4.1, https://purl.org/becyt/ford/4, TRICHODERMA

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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