AbstractLipid composition was studied in different white muscle zones (ventral, dorsal and tail) of wild and farmed blackspot seabream (Pagellus bogaraveo). The study was complemented by moisture, trimethylamine oxide (TMAO) and trace mineral determinations. Farmed fish muscle showed higher lipid and triacylglycerol contents, but lower values for moisture, TMAO and α‐tocopherol than its wild fish counterpart; no differences could be observed between both kinds of fish for the phospholipid, sterol and free fatty acid contents. When compared to wild fish, a higher saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acid (PUFA) content was obtained in farmed fish, while lower values could be observed for the n‐3/n‐6 and 22:6n‐3/20:5n‐3 fatty acid ratios. Most minerals analysed (Cu, Fe, Mn, Se and Zn) showed higher mean values in farmed fish muscle, except for Ca and Mg which provided higher mean contents in wild fish. Concerning the muscle site comparison, greater SFA, MUFA and PUFA contents could be detected in the dorsal zone than in the two other locations both for farmed and wild fish, in accordance with a higher mean lipid content found at this site. Finally, the tail zone showed higher TMAO values than the two other locations.