Sedimentological, paleobiological, mineralogical, and geochemical analyses of a sediment core retrieved on the seafront of Cartagena Bay were performed after high-resolution sampling. A wide series of dates obtained through radiocarbon and amino acid racemization proved that the Holocene record on the analyzed core began at ca. 7300 yr cal BP. Reinterpretation of the marine seismic profiles indicated that the beginning of this transgression was channeled along erosive paleochannels excavated on a coastal plain of, at least, MIS5c age. The Holocene paleoenvironmental evolution consisted of a first period marked by marine conditions with strong inputs from a fan delta linked to the reorganized fluvial network that occurred after the sudden rise of the base level, which caused a growing sedimentary prism. Later, the full marine environment was reflected in the development of a Posidonia- Cymodocea meadow hosting a rich biodiversity of marine species (mollusks, foraminifera, ostracoda). Around 3800 yr cal BP, the area underwent a profound change and a silting process started to alter the conditions, turning the area into a paucispecific brackish marsh environment in which mud deposition was dominant. Since Carthaginian times, arrangement works locally affected the record, allowing the arrival of some marine species due to port work (dredging) undertaken to gain depth and showing anthropic influence.