The aim of this paper was to assess biodiversity among different habitats of an organic farm and the relationships between some soil properties, nematode taxonomic diversity, and soil food web condition. Eight habitats were studied in the farm ponds, ditches, a riparian corridor, hedgerows, and four agricultural fields (mustard, oats, fallow, and legumes). The undisturbed riparian corridor had higher soil NO3- - N and NH4+ - N concentrations, and potentially mineralizable N and higher abundances of bacterivore nematodes and longer food webs. Canonical correlation analysis showed associations between habitats and nematode trophic groups predatory and bacterial-feeding nematodes in the riparian corridor and hedgerows, omnivore nematodes in the ponds and ditches, and fungal-feeding nematodes in the legume field. Soil chemical and physical properties mirrored the aboveground farm patterns and were more similar among habitats that were or had been cultivated, compared to the riparian corridor. Soil food web indices, based on functional analysis of nematode faunal composition, reflected the aboveground landscape heterogeneity. Discriminant analysis indicated that soil food web indices separated the two most disturbed habitats (ponds and tailwater ditches) from the two least disturbed habitats (the riparian corridor and hedgerows). The indices correlated with soil functioning as inferred by soil properties. Abundance of nematode taxa was not associated with aboveground landscape patterns. The complexity of the soil food web may have been influenced by (1) environmental factors that differed between years, (2) different time periods since disturbance in the various habitats, and (3) movement of nutrients and organisms by water flow between habitats in the farmscale. © 2007 Springer-Verlag.