Downloads provided by UsageCountsMulti-laboratory experiment PME11 for the standardization of phosphoproteome analysis
Copyright policy ) Colomé, Nuria; Abián, Joaquín; Aloria, Kerman; Arizmendi, Jesús M.; Barceló-Batllori, Silvia; Braga-Lagache, Sophie; Burlet-Schiltz, Odile; +50 Authors
Colomé, Nuria; Abián, Joaquín; Aloria, Kerman; Arizmendi, Jesús M.; Barceló-Batllori, Silvia; Braga-Lagache, Sophie; Burlet-Schiltz, Odile; Carrascal, Montserrat; Casal, J. Ignacio; Chicano-Gálvez, Eduardo; Chiva, Cristina; Clemente, Luis Felipe; Elortza, Felix; Estanyol, Josep M.; Fernández-Irigoyen, Joaquín; Fernandez-Puente, Patricia; Fidalgo, María José; Froment, Carine; Fuentes, Manuel; Fuentes-Almagro, Carlos; Gay, Marina; Hainard, Alexandre; Heller, Manfred; Hernández, María Luisa; Ibarrola, Nieves; Iloro, Ibon; Kieselbach, Thomas; Lario, Antonio; Locard-Paulet, Marie; Marina, Anabel; Martín, Luna; Morato-López, Esperanza; Muñoz, Javier; Navajas, Rosana; Odena, Maria Antonia; Odriozola, Leticia; Oliveira, Eliandre; Paradela, Alberto; Pasquarello, Carla; Ríos, Vivian de los; Ruiz-Romero, Cristina; Sabidó, Eduard; Sánchez del Pino, Manuel M.; Sancho, Jaime; Santamaría, Enrique; Schaeffer-Reiss, Christine; Schneider, Justine; De la Torre, Carolina; Valero, M. Luz; Vilaseca, Marta; Wu, Shuai; Wu, Linfeng; Ximénez de Embún, Pilar; Canals, Francesc; Corrales, Fernando J.; ProteoRed-ISCIII; EuPA;
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.
Spain, Switzerland, Sweden, Spain
Reproducibility of results, Proteomics, FENÓMENOS Y PROCESOS::fenómenos químicos::fenómenos bioquímicos::fosforilación, enrichment, Proteome, Cellbiologi, Biophysics, Bioinformatics and Computational Biology, Mass-spectrometry, Reproducibilidad de los resultados, 610 Medicine & health, Proteòmica, Biochemistry, ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results, Experiment, Fosforilació, DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica, quality control, Phosphorylation, Epidemiologia, Biokemi, Molecular Biology, PHENOMENA AND PROCESSES::Chemical Phenomena::Biochemical Phenomena::Phosphorylation, Proteoma, Molekylärbiologi, Fosfoproteïnes, Reproducibility of Results, TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::diseño de la investigación epidemiológica::reproducibilidad de los resultados, Cell Biology, Estándares de referencia, Normalització, Reference Standards, Phosphoproteins, Standardization, Proteómica, Bioinformatik och beräkningsbiologi, DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics, Phosphoproteome, Reference standards, Laboratories
Reproducibility of results, Proteomics, FENÓMENOS Y PROCESOS::fenómenos químicos::fenómenos bioquímicos::fosforilación, enrichment, Proteome, Cellbiologi, Biophysics, Bioinformatics and Computational Biology, Mass-spectrometry, Reproducibilidad de los resultados, 610 Medicine & health, Proteòmica, Biochemistry, ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results, Experiment, Fosforilació, DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica, quality control, Phosphorylation, Epidemiologia, Biokemi, Molecular Biology, PHENOMENA AND PROCESSES::Chemical Phenomena::Biochemical Phenomena::Phosphorylation, Proteoma, Molekylärbiologi, Fosfoproteïnes, Reproducibility of Results, TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::diseño de la investigación epidemiológica::reproducibilidad de los resultados, Cell Biology, Estándares de referencia, Normalització, Reference Standards, Phosphoproteins, Standardization, Proteómica, Bioinformatik och beräkningsbiologi, DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics, Phosphoproteome, Reference standards, Laboratories
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