Agricultural production delivers enormous waste biomass materials, which have very low values for industries, but contributes significant environmental impact on carbon emission. This study was aimed to identify cost-effective chemical technologies to enhance bio-accessibility of cellulose-based agricultural biomass materials, which can be used as carbohydrates for fermentation process. Three chemical methods alkaline, dilute acid and ammonia soaking were investigated for the pretreatment of oat husk, which was used as a cellulose biomass model. Comparison studies of the pretreatment methods were carried out in a series of bench scale tests and biochemical analysis. These chemical pretreatment methods were evaluated by enzymatically hydrolysing the treated oat husk and estimating the mount of glucose produced in the hydrolysate. Experimental results revealed these pretreatment methods are promising for enhancing the enzymatic accessibility of the cellulose biomass materials. Alkaline pretreatment method appeared to demonstrate the best results in terms of enzymatic hydrolysis and glucose yield.