Almond (Prunus dulcis), a stone fruit belonging to a family Rosaceae (rose) is broadly cultivated for ornament and fruit. Within this genus, the almond is very much associated with the peach, and these two fruits share the same subgenus the Amygdalus. About 430 species are spread all through the northern temperate regions of the world. The Mediterranean climate region of the Middle East like Turkey and Pakistan eastward to Syria is native to the almond and its related species. Almond is one of the ancient fruit trees known to the Asian as well as European regions with the most primitive proof of cultivation dating about 2000 B.C. Prunus dulcis (Almond) is a nutrient-loaded nut crop. Almond possesses a great genetic diversity due to the genetically controlled self-incompatibility system which can be estimated by a morphological characteristic including molecular markers and isoenzymes with a wide range of marker techniques. Simple sequence repeats (SSR) involving RFLP or SNP are the most commonly used molecular techniques among the DNA-based molecular symbols. Particular agronomic characters, e.g. kernel bitterness or self-compatibility can also be traced by these molecular markers. The direct association between the level of diversity and the basis of the germplasm cannot be understood by the studies of genetic diversity. Genetic diversity cannot be seriously lost by self-compatibility in almonds. The breeding, conservation, and cultivation of wild-growing almonds may similarly advantageous after the genetic diversity research studies (especially those applying molecular markers).