The rescue of commercial species is a priority for biotechnology to increase multiplication rate and develop reforestation. Such programmes can be carried out simultaneously with the development of the country to mantain the ecosystem natural equilibrium. Teak (Tectona grandis L.) is a forest tree of high commercial and ecological value because of its rapid growth and wood quality. Teak is a specie with problems in the propagation as a result of its high genetic variability. For this reason, alternatives to produce propagules have been developed. With the aim to obtain plants regeneration from this were used shoot tips and flowers immature of mature tree explants and cotyledons from seed. Different thidiazuron concentrations was tested for callus formation and cytokinins for plants regeneration. Nodular calli was obtained from shoot tips, cotyledons and flowers immature. The shoots regeneration was achieved in calli from shoot tips.