Cotton plant is produced with seeds. However, its genetic structure is suitable for vegetative production. In this study, rooting method used to propagate cotton plant as vegetative. Experiment was conducted in climate chamber at East Mediterranean Transitional Zone Agricultural Research Institute in 2020. Stems of Askabat 100 (G. barbadenseL.), Stoneville 468 (G. hirsutumL.) cultivars and their F1hybrid were used as plant materials while 2000 ppmIBA (Indole butyric acid) concentration used as chemical materials. As a result of the study, it was determined that cotton genotypes rooted and formed shoots in different rates. Moreover, F1hybrid produced more clones than their parents, followed by Askabat 100 and Stoneville 468. Also, F1hybrid were showed that superior heterotic effects as heterosis and heterobeltiosis in term of investigated properties. This result showed that cotton cultivars and hybrids can be propagation as clone using by rooting,and these clones could be used as rootstocks again in clone production.