Camu-camu is an Amazonian species with fruits that have high a concentration of ascorbic acid, a feature of interest to the food and pharmacological industries. However, for cultivation, studies are needed on seedling production and the use of alternative substrates. The objective of the present study was to develop camu-camu seedlings using different alternative substrates. For seedlings formation, seeds were removed from fruits collected from plants on the Urubu River in Boa Vista-RR, Brazil. A completely randomized experimental design was used, with ten substrates, five replications and five plants per replication. The treatments consisted of: [T1 - Soil; T2 - standard substrate: 3 parts sand soil + 1, T3 - commercial substrate: organoamazon®, T4 – standard substrate +75 % manure (E), T5 - standard substrate +50 % E; T6 - standard substrate + 25 % E, T7 - standard substrate +75 % rice hulls (C), T8 - standard substrate + 50% C; standard substrate T9 = 25% + C; T10 standard substrate +25% E + 25 % C]. The following characteristics were evaluated: plant height (cm), diameter (mm), shoot and root dry matter (g), root length (cm) and number of leaves. The substrate consisting of standard soil and sand at the ratio 3:1 gave favorable results for all the characteristics. Substrates with increasing proportions of rice hulls and manure did not improve the camu-camu tree seedling development.