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Anales del Jardín Botánico de Madrid
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Characterization of mycorrhizal fungi of the genus Tulasnella (Tulasnellaceae, Basidiomycota) in the genus of orchids Bletia from Barranca del Cupatitzio Natural Reserve, Mexico

توصيف الفطريات الجذرية الفطرية من جنس Tulasnella (Tulasnellaceae، Basidiomycota) في جنس بساتين الفاكهة Bletia من محمية Barranca del Cupatitzio الطبيعية، المكسيك
Authors: María de los Ángeles Beltrán-Nambo; Juan Carlos Montero-Castro; Miguel Martínez-Trujillo; Rafael Salgado-Garciglia; J. T. Otero-Ospina; Yazmin Carreón-Abud;

Characterization of mycorrhizal fungi of the genus Tulasnella (Tulasnellaceae, Basidiomycota) in the genus of orchids Bletia from Barranca del Cupatitzio Natural Reserve, Mexico

Abstract

The goal of this study was the identification of mycorrhizal fungi associated with three terrestrial orchids of the genus Bletia Ruiz & Pav.: B. roezlii Rchb. f., B. purpurata A.Rich., and B. punctata Lex., in Barranca del Cupatitzio Natural Reserve - Michoacán, México - . Thirty-nine strains were isolated and morphologically characterized. Nine strains were selected from the molecular analysis. Bletia punctata, an endemic species of Mexico, showed the lowest variability in mycorrhizal fungi. Morphological analysis showed that 39 isolated strains belong to the ‘Rhizoctonia-like fungal complex’. According with the tree of Euclidian distances generated by the analysis WARD, all isolates were included into four subgroups, all related to the genus Tulasnella J.Schröt - which belongs to the ‘Rhizoctonia-like fungal complex’?. Molecular and phylogenetic analysis of the nine selected strains corroborated the results of the morphological study: the sequences obtained were clustered in four subclades related to species of Tulasnella. Our results indicate that a single species of Bletia from a single locality can be associated with different species of mycorrhizal fungi, at least during the adult stage and that the combination of morphological and molecular analyses is a good tool to identify orchid mycorrhizal fungi.

Keywords

Rhizoctonia-like fungal complex, Tulasnella, tulasnella, Plant Science, Biochemistry, Gene, Molecular Systematics of Gesneriaceae and Allies, Rhizoctonia, Agricultural and Biological Sciences, Biochemistry, Genetics and Molecular Biology, its, Molecular Biology, Biology, Ecology, Evolution, Behavior and Systematics, complejo Rhizoctonia, Genus, Basidiomycota, Rhizoctonia solani, Botany, Life Sciences, orchid-fungal partnership, orquídeas terrestres, Strigolactone Signaling in Plant Interactions, terrestrial orchids, Mycorrhizal Fungi, QK1-989, rhizoctonia-like fungal complex, Impact of Pollinator Decline on Ecosystems and Agriculture, ITS, Asociación orquídea-hongo, Phylogenetic tree

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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