Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)

descriptionPublicationkeyboard_double_arrow_right Article 11 Feb 2014 Brazil English Publisher:WileyJournal:Applications in Plant Sciences, volume 2 (issn: 2168-0450, eissn: 2168-0450,

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Authors: Manoel, Ricardo de Oliveira; Freitas, Miguel L. M.; Barreto, Mariana A.; Moraes, Mario Luis Teixeira de; Souza, Anete P.; Sebbenn, Alexandre M.;

doi: 10.3732/apps.1300084

pmid: 25202610

pmc: PMC4103104

handle: 11449/117525

Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)

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Abstract

• Premise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies.• Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.• Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana.

Country

Brazil

Related Organizations

State University of Campinas
Brazil
Universidade Estadual Paulista - UNESP
Brazil
Sao Paulo State University
Brazil

Keywords

Genipa americana, QH301-705.5, 590, Botany, population genetics, Rubiaceae, microsatellite markers, tree species, conservation genetics, QK1-989, Biology (General)

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