TWO new species of frogs of the genus Colostethus from the Venezuelan highlands are described. Colostethus duranti, from the paramo and subparamo La Culata, Estado Merida, is distinguished from all other Venezuelan Colostethus by the presence of a transversal sacral flap above the vent and by its white and light blue dotted belly. Colostethus serranus from the cloud- forest area of El Morro, Estado Merida, is distinguished by the lemon-yellow color of the belly and the pink to copper color of the ventral parts of the thighs.