This research aims to evaluate psychometric properties of a new instrument for measuring vocational interest: Padjadjaran Interest Inventory (PII). There were 2,648 participants in this study, consisting of workers, high school, and university students, with gender proportion of 1,014 (38.3%) males and 1,634 (61.7%) females. This research used descriptive statistic test, t test and MANOVA for gender differences, reliability coefficients and validity evidence by using confirmatory factor analysis (CFA). The results showed that PII has a good psychometric properties: it has good reliability and valid internal structure; it is standardized by gender; and it is applicable for large groups with relative ease. PII can be used for career exploration. Limitation of this study was discussed for future research.