Two field experiments were conducted at the Experimental Station Farm, Faculty of Agriculture, Mansoura University, Egypt, during 2014/2015 and 2015/2016 seasons to estimate the effect of organic (compost and compost tea), mineral (100%, 75% and 50% from recommended dose of NPK) and bio-fertilization (without, mixture of bacteria with fungi and EM) as well as their interactions on growth, yield and quality of broccoli plants. Data which obtained in this experiment indicated that organic treatments gave the highest means of vegetative growth parameters, total yield and quality of broccoli heads were recorded from plants which fertilized with compost compare to compost tea except total carbohydrates and regarding to mineral fertilization, broccoli plants which fertilized with 75% NPK from recommended dose gave the maximum means of all studied characters in both seasons. Moreover bio-fertilizers, the highest values of all parameters were resulted from plants treated with EM followed by bio-fertilizer mixture treatment in both seasons. It could be recommended that fertilization of broccoli plants with compost (4 ton/fed.), 75% NPK from recommended dose and inoculation with EM (10 ml/plant) in would improve broccoli plants productivity and quality.